Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Hnf1b-cre/ERT2)1Jfer
Name:	transgene insertion 1, Jorge Ferrer
MGI ID:	MGI:4420452
Synonyms:	Hnf1bCreER
Transgene:	Tg(Hnf1b-cre/ERT2)1Jfer  Location: unknown
Alliance:	Tg(Hnf1b-cre/ERT2)1Jfer page

Strain of Origin:

C57BL/6J x SJL

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Tg(Hnf1b-cre/ERT2)1Jfer expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Hnf1b (MGI:98505)   Mutation details: A BAC clone containing the Hnf1b locus (RP23-304H7) was modified by replacing exon 1 with a targeting vector containing an Hnf1b promoter fragment, cre/ERT2, IRES, d2EGFP (a destabilized variant of EGFP), the b-globin polyadenylation site, a FRT/neo/FRT cassette, and an intron 1 fragment that flanks exon 1 of Hnf1b. The targeting vector was electroporated into EL250 cells and homologous recombination was induced. Correctly targeted clones were treated to activate inducible Flp recombinase, removing the FRT/neo/FRT cassette. The recombined BAC was digested, linearized and microinjected into pronuclei of C57BL6/SJL eggs. 10 lines were generated, and after crossing to Rosa26 reporter mice, one line carrying a single copy of the transgene was maintained (after characterization of 4 lines exhibiting similar reporter recombination patterns.) (J:156016)

Activity:	Tissue activity of this recombinase allele
Driver:	Hnf1b (mouse) Summary of all recombinase alleles driven by Hnf1b. Alliance mouse Hnf1b gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:156016 Solar M, et al., Pancreatic exocrine duct cells give rise to insulin-producing beta cells during embryogenesis but not after birth. Dev Cell. 2009 Dec;17(6):849-60
All:	15 reference(s)