Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Nos3tm1Gdk
Name:	nitric oxide synthase 3, endothelial cell; targeted mutation 1, Axel Godecke
MGI ID:	MGI:2159003
Synonyms:	eNOS-
Gene:	Nos3  Location: Chr5:24569808-24589472 bp, + strand  Genetic Position: Chr5, 11.32 cM
Alliance:	Nos3tm1Gdk page

Germline Transmission:	Earliest citation of germline transmission: J:46061
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A genomic fragment containing exons 24 and part of 25 was replaced with a neomycin selection cassette. The deleted sequences encode the NADPH binding sites. Western blot analysis on extracts of heart, lung, kidney and liver, and immunohistochemistry experiments on homozygous mice confirmed that no stable protein was expressed from this allele. (J:46061, J:62375)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:	56 strains or lines available

Original:	J:46061 Godecke A, et al., Coronary hemodynamics in endothelial NO synthase knockout mice. Circ Res. 1998 Feb 9;82(2):186-94
All:	30 reference(s)