Id1tm1Bene
Targeted Allele Detail
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| Symbol: |
Id1tm1Bene |
| Name: |
inhibitor of DNA binding 1, HLH protein; targeted mutation 1, Robert Benezra |
| MGI ID: |
MGI:4366905 |
| Synonyms: |
Id1V, Id1VenusYFP |
| Gene: |
Id1 Location: Chr2:152578171-152579330 bp, + strand Genetic Position: Chr2, 75.41 cM
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| Alliance: |
Id1tm1Bene page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:154023
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| Parent Cell Line: |
CY2.4 (ES Cell)
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| Strain of Origin: |
B6(Cg)-Tyrc-2J
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| Allele Type: |
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Targeted (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The coding sequence of the Venus yellow fluorescent protein was linked to the 3' end of the Id1 coding sequence with a sequence for a 10-amino acid flexible linker (GSAGSAAGSG) to create an Id1/YFP fusion protein. Following this insertion, the endogenous 3' untranslated region was unperturbed and utilized as the polyadenylation sequence. The FRT-Neo-FRT cassette for positive selection was inserted 477 bp downstream of the modified Id1 gene. CY2.4 ES cells (albino C57BL/6 derivation) were used for targeting. Southern blot positive ESC clones were injected into co-isogenic B6J blastocysts. Positive chimeras were bred to albino B6J females. Albino pups were genotyped by PCR, and positive pups were bred to B6 ACTB::FLPE mice (Artemis Pharamaceuticals) to excise the Neo cassette in vivo.
(J:154023)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Id1 Mutation: |
8 strains or lines available
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This mutant allele can substitute for the wild-type allele (i.e. mice that are of Id1(Venus/Venus); Id3(-/-) genotype were viable and normal at weaning).
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| Original: |
J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26 |
| All: |
8 reference(s) |
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Analysis Tools
