Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Id1tm2(cre/ERT2)Bene
Name:	inhibitor of DNA binding 1, HLH protein; targeted mutation 2, Robert Benezra
MGI ID:	MGI:4366863
Synonyms:	Id1IRES-creERT2
Gene:	Id1  Location: Chr2:152578171-152579330 bp, + strand  Genetic Position: Chr2, 75.41 cM
Alliance:	Id1tm2(cre/ERT2)Bene page

Germline Transmission:	Earliest citation of germline transmission: J:154023
Parent Cell Line:	CY2.4 (ES Cell)
Strain of Origin:	B6(Cg)-Tyrc-2J

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Id1tm2(cre/ERT2)Bene expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Id1 (MGI:96396)   Mutation details: An encephalomyocarditis virus internal ribosomal entry site (EMCV IRES) and a tamoxifen-inducible recombinase cre/ERT2 coding sequence were inserted after the Id1 gene coding sequence. An FRT-Neo-FRT cassette was inserted after the IRES-cre/ERT2 sequence and before the 3' untranslated region. The Id1 protein and creERT2 protein are translated from a bicistronic messenger RNA with the endogenous polyadenylation sequence. CY2.4 ES cells (albino C57BL/6 derivation) were used for targeting. Southern blot positive clones were injected into co-isogenic B6J blastocyts. Positive chimeras were bred to albino B6J females. Albino pups were genotyped by PCR, and positive pups were bred to B6 ACTB::FLPE mice (Artemis Pharamaceuticals) to excise the Neo cassette in vivo. (J:154023)

Activity:	Tissue activity of this recombinase allele
Driver:	Id1 (mouse) Summary of all recombinase alleles driven by Id1. Alliance mouse Id1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Id1 Mutation:	8 strains or lines available

Original:	J:154023 Nam HS, et al., High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009 Nov 6;5(5):515-26
All:	2 reference(s)