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Runx1tm1Yg
Targeted Allele Detail
Summary
Symbol: Runx1tm1Yg
Name: runt related transcription factor 1; targeted mutation 1, Yoram Groner
MGI ID: MGI:3760788
Synonyms: P2neo, P2neo, Runx1P2neo
Gene: Runx1  Location: Chr16:92398354-92622962 bp, - strand  Genetic Position: Chr16, 53.7 cM
Alliance: Runx1tm1Yg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:125830
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation details: A loxP-flanked neomycin resistance cassette was inserted, in opposite transcriptional orientation, at nucleotide 27675861 of the mouse Chr 16 genomic sequence (NCBI Ref Seq NT-039625), ~1 kb upstream of the transcription initiation site from the P2 (more proximal) transcriptional promoter in exon 2. In E16.5 kidney and P1.5 tongue, where transcription normally occurs from both the P1 (more distal) and P2 promoters, only P1-derived transcripts are detected by RT-PCR in homozygous mutant tissues; E16.5 stomach epithelium, which normally employs only the P2 promoter, expresses no Runx1 transcripts in homozygous mutants. In thymus, RT-PCR and immunohistochemistry confirm the lack of mRNA and protein expression in mutant embryos at E15.5 and its gradual recovery by E16.5-E17.5 as the transition from the P2 to the P1 promoter occurs. (J:125830)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Runx1 Mutation:  35 strains or lines available
Notes
Removal of the neo cassette by cre-mediated recombination following crosses with either Tg(Pgk1-cre)1Lni or Tg(EIIa-cre)C5379Lmgd mice resulted in a reversion back to a phenotype indistunguishable from wild-type phenotype (Runx1P2loxP or P2loxP).
References
Original:  J:125830 Pozner A, et al., Developmentally regulated promoter-switch transcriptionally controls Runx1 function during embryonic hematopoiesis. BMC Dev Biol. 2007;7:84
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory