Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cd79btm168Mnz
Name:	CD79B antigen; targeted mutation 168, Michel C Nussenzweig
MGI ID:	MGI:3720567
Synonyms:	IgBISceI
Gene:	Cd79b  Location: Chr11:106202167-106205388 bp, - strand  Genetic Position: Chr11, 68.89 cM
Alliance:	Cd79btm168Mnz page

Germline Transmission:	Earliest citation of germline transmission: J:148011
Parent Cell Line:	CY2.4 (ES Cell)
Strain of Origin:	B6(Cg)-Tyrc-2J

Allele Type:    Targeted (Not Applicable) Mutation:    Insertion   Mutation details: A targeting vector containing a self-excising lox-P flanked neomycin resistance cassette was used to introduce an 18 bp I-SceI (S. cerevisiae) endonuclease recognition site (TAGGGATAACAGGGTAAT) in intron 2 of the gene. The construct was electroporated into CY2.4 albino C57BL/6J-Tyrc-2J-derived embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6J-Tyrc-2J mice to allow for coat-color screening of germline transmission. White pups carrying the mutation were then crossed to C57BL/6 background EIIA Cre mice to excise the neomycin cassette, leaving one loxP site in intron three of the gene. Crosses with C57BL/6 were carried out to remove the Cre transgene. (J:148011)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cd79b Mutation:	36 strains or lines available

Original:	J:148011 Nussenzweig M, Generation of a knock-in allele of Cd79b. MGI Direct Data Submission. 2009;
All:	1 reference(s)