Gt(ROSA)26Sortm1(Stat3,EGFP)Cgn
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm1(Stat3,EGFP)Cgn |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, University of Cologne |
| MGI ID: |
MGI:3655484 |
| Synonyms: |
R26LSL-Stat3C, R26-Stat3cfl-STOP, Stat3-CFL |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm1(Stat3,EGFP)Cgn page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:109583
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| Parent Cell Line: |
Other (see notes) (ES Cell)
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| Strain of Origin: |
mixed
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| Allele Type: |
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Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Gt(ROSA)26Sortm1(Stat3,EGFP)Cgn expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Stat3 (MGI:103038) |
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Mutation details: ES cells from Ighg1tm1(IRES-cre)Cgn heterozygous mice were targeted at the ROSA26 locus with a conditional transgene encoding a constitutively active form of the STAT-3 protein proceded by a floxed STOP cassette and followed by an IRES-EGFP cassette. Upon SRBC immunization, STAT-3c expression is detected in the majority of germinal centers and IgG1 memory B cells by EGFP expression.
(J:109583)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gt(ROSA)26Sor Mutation: |
944 strains or lines available
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ES cell lines were established from Ighg1tm1(IRES-cre)Cgn heterozygous mice on the (C57BL/6 x BALB/c)F1 genetic background. Such ES cells can be used for further genetic manipulation and then injected into tetraploid blastocysts to obtain cloned mice for analysis.
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| Original: |
J:109583 Casola S, et al., Tracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting. Proc Natl Acad Sci U S A. 2006 May 9;103(19):7396-401 |
| All: |
2 reference(s) |
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Analysis Tools
