Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Plcb4Gt(pU21)58Imeg
Name:	phospholipase C, beta 4; gene trap 58, Institute of Molecular Embryology and Genetics
MGI ID:	MGI:3630388
Synonyms:	GtAyu21-58, Gt(Ayu21)58Imeg
Gene:	Plcb4  Location: Chr2:135500931-135856513 bp, + strand  Genetic Position: Chr2, 66.64 cM
Alliance:	Plcb4Gt(pU21)58Imeg page

Mutant Cell Line:	Ayu21-58
Germline Transmission:	Earliest citation of germline transmission: J:109358
Parent Cell Line:	KTPU10 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Gene trapped Mutation:    Insertion of gene trap vector     Vector: pU-21   Mutation details: The "promoter trap" gene trap vector pU21 contains 1.2 kb of intron 1 and part of exon 2, including the splice acceptor, from the mouse engrailed 2 gene (En2) followed by the Betageo beta-galactosidase/neomycin resistance fusion gene with the SV40 and mouse Pgk1 polyadenylation signals. Three stop codons occur in the exon sequence upstream of and in frame with the Betageo translation initiation codon. loxP and FRT sites used for 5' RACE analysis and for in vitro exchange of other sequences for Betageo are present. (J:109358) Inheritance:    Not Specified Sequence Tags:  Sequence tag details (1 tag) Tag ID GenBank ID Method Tag Location (trapped strand)* Select Ayu21-58 AB187246 (MGI Seq Detail) 5' RACE Chr2:135499123-135499429 bp (+) * GRCm39 (strand of gene trap mutagenesis) Selected Tags: download BLAST at NCBI Genome Context:  Genome Browser view of this mutation 95 gene trap insertions have trapped this gene View all gene trap sequence tags in this region

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Plcb4 Mutation:	130 strains or lines available

Original:	J:109358 Database for Exchangeable Gene Trap Clones, Information obtained from the Database for Exchangeable Gene Trap Clones (EGTC), an official database of the Gene Trap Project of the Institute of Molecular Embryology and Genetics and the Institute of Resource Development and Analysis, Kumamoto University, Japan. Unpublished. 2006;
All:	3 reference(s)