Aco1tm1Mwh
Targeted Allele Detail
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| Symbol: |
Aco1tm1Mwh |
| Name: |
aconitase 1; targeted mutation 1, Matthias W Hentze |
| MGI ID: |
MGI:3613021 |
| Synonyms: |
Irp1- |
| Gene: |
Aco1 Location: Chr4:40143081-40198338 bp, + strand Genetic Position: Chr4, 20.24 cM
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| Alliance: |
Aco1tm1Mwh page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:104538
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| Parent Cell Line: |
E14 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Conditional ready, No functional change) |
| Mutation: |
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Insertion
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Mutation details: A gene trap cassette containing an IRESbetageo was introduced into the second intron of the locus. Insertion of the cassette is expected to abrogate the expression of functional protein. The Betageo was flanked with frt sites to allow conditional inactivation of the locus and the frame-shifting exon 3 is flanked with loxP sites. Transcript was not detected by Northern blot of various mutant organs. Protein was detected at 5% of wild-type levels by Western blot analysis, suggesting that the gene trap construct is leaky.
(J:104538)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Aco1 Mutation: |
51 strains or lines available
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| Original: |
J:104538 Galy B, et al., Targeted mutagenesis of the murine IRP1 and IRP2 genes reveals context-dependent RNA processing differences in vivo. RNA. 2004 Jul;10(7):1019-25 |
| All: |
3 reference(s) |
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Analysis Tools
