Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gja1tm5(cre/ERT)Kwi
Name:	gap junction protein, alpha 1; targeted mutation 5, Klaus Willecke
MGI ID:	MGI:2676327
Synonyms:	Cx43Cre-ER(T), Gja1tm2(cre/ESR1)Kwi, Gja1tm5(cre/ESR1)Kwi
Gene:	Gja1  Location: Chr10:56253297-56266519 bp, + strand  Genetic Position: Chr10, 28.64 cM
Alliance:	Gja1tm5(cre/ERT)Kwi page

Germline Transmission:	Earliest citation of germline transmission: J:85065
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1b-m3

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Gja1tm5(cre/ERT)Kwi expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Gja1 (MGI:95713)   Mutation details: The coding region was replaced with a construct encoding a tamoxifen dependent cre recombinase. The cre recombinase coding region was joined to sequence encoding the ligand binding domain of the human estrogen receptor with a glycine to arginine missense mutation at position 521. This substitution enables the domain to bind to tamoxifen while ablating the estradiol binding ability. Tamoxifen treatment permits the translocation of the cre hybrid protein to the nucleus. (J:85065)

Activity:	Tissue activity of this recombinase allele
Driver:	Gja1 (mouse) Summary of all recombinase alleles driven by Gja1. Alliance mouse Gja1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gja1 Mutation:	59 strains or lines available

Original:	J:85065 Kretz M, et al., Altered connexin expression and wound healing in the epidermis of connexin-deficient mice. J Cell Sci. 2003 Aug 15;116(Pt 16):3443-52
All:	18 reference(s)