Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Nos3tm1Unc
Name:	nitric oxide synthase 3, endothelial cell; targeted mutation 1, University of North Carolina
MGI ID:	MGI:1857229
Synonyms:	ecNOS-, eNOS-, eNOSKO, NOS3-
Gene:	Nos3  Location: Chr5:24569808-24589472 bp, + strand  Genetic Position: Chr5, 11.32 cM
Alliance:	Nos3tm1Unc page

Germline Transmission:	Earliest citation of germline transmission: J:36559
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A 1.2 kb neomycin cassette replaced 129 bp of exon 12 of the gene. This disrupted the calmodulin binding site of the protein and introduced a premature stop codon into the transcripts. Immunohistochemisty of heart and kidney sections from homozygous mutant mice confirmed that no detectable encoded protein was present. (J:36559)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	5 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	35 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 6 strains available      Cell Lines: 0 lines available
Carrying any Nos3 Mutation:	56 strains or lines available

Original:	J:36559 Shesely EG, et al., Elevated blood pressures in mice lacking endothelial nitric oxide synthase. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13176-81
All:	433 reference(s)