Analysis Tools
digestive/alimentary system
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• the number of cleaved CASPASE-3-positive small intestine crypt cells is increased at 0, 2, 4, and 7 days after beta-napthoflavone (betaNF) treatment
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• the number of BrdU-positive small intestine crypt cells is reduced at day 2 but increased by day 4 post-betaNF treatment
• similarly, the number of BrdU/Krt8-double positive crypt cells per small intestine length is reduced at day 2 but increased by day 4 post-betaNF treatment
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• at day 2 after the last of 5 consecutive beta-napthoflavone (betaNF) injections, small intestine architecture is severely disrupted, with fewer and smaller crypts and shorter, hypocellular and progressively disorganized villi
• however, by day 4 after the last betaNF injection, ORC1 protein levels are restored to nearly normal levels and a 4- to 5-day repopulation period ensues with enhanced DNA replication, progenitor cell proliferation and crypt hypertrophy such that crypt-villus integrity is fully restored at day 7 post-treatment
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• at day 2 post-betaNF treatment, fewer and smaller crypts are observed in the small intestine
• however, crypt integrity is fully restored at day 7 post-betaNF treatment
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• at day 2 post-betaNF treatment, small intestine villi are shorter, hypocellular and progressively disorganized
• however, villus integrity is fully restored at day 7 post-betaNF treatment
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• small intestine villi are shorter at day 2 but not at day 7 post-betaNF treatment
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endocrine/exocrine glands
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• at day 2 post-betaNF treatment, fewer and smaller crypts are observed in the small intestine
• however, crypt integrity is fully restored at day 7 post-betaNF treatment
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cellular
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• the number of cleaved CASPASE-3-positive small intestine crypt cells is increased at 0, 2, 4, and 7 days after beta-napthoflavone (betaNF) treatment
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• the number of BrdU-positive small intestine crypt cells is reduced at day 2 but increased by day 4 post-betaNF treatment
• similarly, the number of BrdU/Krt8-double positive crypt cells per small intestine length is reduced at day 2 but increased by day 4 post-betaNF treatment
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• DNA replication is enhanced during the 4- to 5-day repopulation period that follows after acute disruption of intestinal integrity
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homeostasis/metabolism
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• DNA replication is enhanced during the 4- to 5-day repopulation period that follows after acute disruption of intestinal integrity
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