Phenotypes Associated with This Genotype

Genotype
MGI:7333158

• Allelic Composition: Del(6Spam1-Hyal5)1Eki/Del(6Spam1-Hyal5)1Eki
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:294353 )

♂N • male mice show no apparent defects in spermatogenesis, sperm maturation or ejaculation relative to control males

decreased litter size ( J:294353 )

♂ • when sexually mature males are bred with wild-type females for >2 months, mean litter size is significantly smaller than in control males

reduced male fertility ( J:294353 )

♂ • males produce significantly fewer offspring than control males, indicating severe male subfertility

♂ • however, formation of copulation plugs in mated females is normal

impaired fertilization ( J:294353 )

♂ • in vitro, epididymal sperm show impaired ability to penetrate the cumulus-oocyte complex (COC) even after adhering to the COC surface

decreased fertilization frequency ( J:294353 )

♂ • in vitro fertilization (IVF) rate is significantly reduced when cumulus-intact eggs are inseminated with capacitated cauda epididymal sperm

♂ • no decrease in IVF rate is observed when COC-free zona pellucida-intact eggs are used

♂ • sperm fertilization ability is restored by adding purified human sperm hyaluronidase to the IVF medium

abnormal sperm physiology ( J:294353 )

♂ • in an in vitro cumulus-oocyte complex (COC) dispersion assay, sperm show defective COC dispersal due to loss of sperm hyaluronidase activity responsible for hydrolyzing hyaluronic acid in the extracellular matrix of cumulus cells

abnormal sperm motility ( J:294353 )

♂ • in an in vitro sperm motility assay, sperm move slowly in the presence of 0.4% hyaluronic acid in TYH medium

cellular

abnormal sperm motility ( J:294353 )

♂ • in an in vitro sperm motility assay, sperm move slowly in the presence of 0.4% hyaluronic acid in TYH medium