Analysis Tools
reproductive system
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• best grown oocytes from 3-week-old females are smaller in diameter, contain a germinal vesicle (GV) with unclear boundaries and aggregate dark cytoplasmic granules
• oocyte transition from the nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) type is inhibited; oocyte development is arrested in the NSN chromatin configuration, indicating impaired nucleolar maturation
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• ovaries show complete oocyte loss at 5 months of age
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• at 8 weeks of age, ovaries are devoid of corpora lutea
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• at 3 weeks of age, ovaries contain fewer growing follicles than those in control females
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• at 3 and 8 weeks of age, ovaries contain higher numbers of primary follicles than control ovaries
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• at 8 weeks of age, ovaries contain fewer primordial follicles than control ovaries
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• at 3 weeks of age, the number of secondary follicles with multilayer granulosa cells is significantly lower than in control ovaries
• however, the number of bilayer secondary follicles is similar to that in control ovaries
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• at 8 weeks of age, ovaries are devoid of follicles containing more than two layers of granulosa cells
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• by 5 months of age, no ovarian follicles are detected
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• 8-week-old females lack follicles beyond the secondary follicle stage
• by 5 months of age, ovaries show complete oocyte and follicle loss
• in culture, secondary follicles containing two layers of granulosa cells from P21 females are arrested at the secondary stage and subsequently degenerate without developing to the antral stage
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small ovary
(
J:302700
)
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• at 3 and 8 weeks of age, ovaries are smaller than in control females
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• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age
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• 3-week-old females are unresponsive to PMSG treatment
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• 3-week-old females fail to ovulate after PMSG plus hCG treatment
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• when 6-week-old females were mated with wild-type males for 5 months, no pups were born
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endocrine/exocrine glands
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• at 8 weeks of age, ovaries are devoid of corpora lutea
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• at 3 weeks of age, ovaries contain fewer growing follicles than those in control females
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• at 3 and 8 weeks of age, ovaries contain higher numbers of primary follicles than control ovaries
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• at 8 weeks of age, ovaries contain fewer primordial follicles than control ovaries
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• at 3 weeks of age, the number of secondary follicles with multilayer granulosa cells is significantly lower than in control ovaries
• however, the number of bilayer secondary follicles is similar to that in control ovaries
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• at 8 weeks of age, ovaries are devoid of follicles containing more than two layers of granulosa cells
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• by 5 months of age, no ovarian follicles are detected
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• 8-week-old females lack follicles beyond the secondary follicle stage
• by 5 months of age, ovaries show complete oocyte and follicle loss
• in culture, secondary follicles containing two layers of granulosa cells from P21 females are arrested at the secondary stage and subsequently degenerate without developing to the antral stage
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small ovary
(
J:302700
)
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• at 3 and 8 weeks of age, ovaries are smaller than in control females
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• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age
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cellular
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• at 3 weeks of age, the % of EU-positive oocytes is significantly higher than in control females, consistent with impaired NSN-SN transition; however, the intensity of EU signals in NSN oocytes is comparable to that in control oocytes, suggesting that global transcription activity is unaffected
• further analysis showed that growing oocytes show impaired 18S rRNA processing activity leading to 28S rRNA accumulation
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• best grown oocytes from 3-week-old females are smaller in diameter, contain a germinal vesicle (GV) with unclear boundaries and aggregate dark cytoplasmic granules
• oocyte transition from the nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) type is inhibited; oocyte development is arrested in the NSN chromatin configuration, indicating impaired nucleolar maturation
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• ovaries show complete oocyte loss at 5 months of age
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• fluorescent staining with L-homopropargylglycine (HPG, an analog of methionine) showed reduced protein synthesis activity in both growing NSN-type and fully-grown SN-type oocytes isolated from 3-week-old females relative to control oocytes
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homeostasis/metabolism
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• fluorescent staining with L-homopropargylglycine (HPG, an analog of methionine) showed reduced protein synthesis activity in both growing NSN-type and fully-grown SN-type oocytes isolated from 3-week-old females relative to control oocytes
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mortality/aging
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• complete oocyte and follicle loss results in premature ovarian failure at 5 months of age
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