Phenotypes Associated with This Genotype

Genotype
MGI:7261374

• Allelic Composition: Gt(ROSA)26Sor^{tm1.1(CAG-COX8A/Dendra2)Dcc}/Gt(ROSA)26Sor⁺; Mff^{Gt(AZ0438)Wtsi}/Mff^{Gt(AZ0438)Wtsi}
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal sperm connecting piece morphology ( J:301349 )

♂ • >5% of caudal epididymidal sperm contain kinks in the neck

abnormal sperm midpiece morphology ( J:301349 )

♂ • >15% of caudal epididymidal sperm contain kinks in the midpiece

abnormal sperm mitochondrial sheath morphology ( J:301349 )

♂ • caudal epididymidal sperm show disjointed mitochondrial sheaths with gaps between adjacent organelles

abnormal sperm principal piece morphology ( J:301349 )

♂ • >40% of caudal epididymidal sperm contain kinks in the principal piece

kinked sperm flagellum ( J:301349 )

♂ • >60% of caudal epididymidal sperm contain kinks in the midpiece, principal piece or neck

abnormal spermatid morphology ( J:301349 )

♂ • most round and elongating spermatids contain tubular mitochondria, unlike in wild-type spermatids where mitochondria are almost always fragmented

asthenozoospermia ( J:301349 )

♂ • caudal epididymidal sperm are significantly less motile than wild-type sperm

decreased fertilization frequency ( J:301349 )

♂ • in an in vitro fertilization assay, cauda epididymidal sperm failed to fertilize any oocytes

cellular

abnormal sperm connecting piece morphology ( J:301349 )

♂ • >5% of caudal epididymidal sperm contain kinks in the neck

abnormal sperm midpiece morphology ( J:301349 )

♂ • >15% of caudal epididymidal sperm contain kinks in the midpiece

abnormal sperm mitochondrial sheath morphology ( J:301349 )

♂ • caudal epididymidal sperm show disjointed mitochondrial sheaths with gaps between adjacent organelles

abnormal sperm principal piece morphology ( J:301349 )

♂ • >40% of caudal epididymidal sperm contain kinks in the principal piece

kinked sperm flagellum ( J:301349 )

♂ • >60% of caudal epididymidal sperm contain kinks in the midpiece, principal piece or neck

abnormal spermatid morphology ( J:301349 )

♂ • most round and elongating spermatids contain tubular mitochondria, unlike in wild-type spermatids where mitochondria are almost always fragmented

abnormal mitochondrial morphology ( J:301349 )

♂ • most round and elongating spermatids contain tubular mitochondria, unlike in wild-type spermatids where mitochondria are almost always fragmented

decreased mitochondrial number ( J:301349 )

♂ • total mito-Dendra2 fluorescence is markedly reduced in the midpiece of epididymidal sperm

asthenozoospermia ( J:301349 )

♂ • caudal epididymidal sperm are significantly less motile than wild-type sperm