Analysis Tools
hematopoietic system
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• under homeostatic conditions, hematopoiesis is normal with no significant changes in liver, spleen and thymus weight, absolute numbers of leukocytes, erythrocytes, platelets and hemoglobin level in peripheral blood, or bone marrow (BM) and spleen cellularity
• normal frequencies of LSKs (Lineage-/Sca1+/c-Kit+), SLAMs (CD150+/CD48-/Lineage-/Sca1+/c-Kit+), B cells (CD19+and B220+), myeloid cells (Mac-1+ and Gr-1+) and T cells (CD3+) in BM
• normal clonogenic potential of hematopoietic stem cells (HSCs) and progenitors
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• after a single dose of 5-fluorouracil (150 mg/kg) treatment, mice show a slightly faster recovery of peripheral leukocytes than wild-type controls
• after two doses 5-fluorouracil, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls
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• Ras palmitoylation is significantly reduced but not completely abolished in bone marrow cells from mutant mice, as shown by an acyl-biotinyl exchange assay
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homeostasis/metabolism
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• after two doses 5-fluorouracil treatment, death is moderately but significantly slower than in wild-type controls
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• after two doses 5-fluorouracil treatment, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls
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immune system
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• after a single dose of 5-fluorouracil (150 mg/kg) treatment, mice show a slightly faster recovery of peripheral leukocytes than wild-type controls
• after two doses 5-fluorouracil, reduction in peripheral leukocyte count is moderately but significantly slower than in wild-type controls
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mortality/aging
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• after two doses 5-fluorouracil treatment, death is moderately but significantly slower than in wild-type controls
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