Phenotypes Associated with This Genotype

Genotype
MGI:6765907

• Allelic Composition: Zfp131^tm1.1Mytk/Zfp131^tm1.1Mytk; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:271921 )

N • differentiation and maturation of CD4 single-positive (SP) and CD8 SP populations within the thymus are normal, with no significant alterations in the numbers of total, double-negative (DN), double-positive (DP), CD4 SP, and CD8 SP thymocytes

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

abnormal T cell subpopulation ratio ( J:271921 )

• increased ratio of memory-phenotype versus naive T cells of either CD4 or CD8 cells in periphery

• many peripheral memory-phenotype T cells retain non-deleted floxed allele suggesting that Znf131-deficient naive T cells released from the thymus are unable to expand to maintain T cell homeostasis in periphery

decreased T cell number ( J:271921 )

• despite normal thymic selection and T cell maturation in the thymus, mice show a significant reduction in absolute numbers of CD3+, CD4+ and CD8+ cells in spleen

• naive T cells released from the thymus are not able to proliferate to maintain naive T cell pool in periphery

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD4+ cells in spleen

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD8+ cells in spleen

hematopoietic system

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

abnormal T cell subpopulation ratio ( J:271921 )

• increased ratio of memory-phenotype versus naive T cells of either CD4 or CD8 cells in periphery

• many peripheral memory-phenotype T cells retain non-deleted floxed allele suggesting that Znf131-deficient naive T cells released from the thymus are unable to expand to maintain T cell homeostasis in periphery

decreased T cell number ( J:271921 )

• despite normal thymic selection and T cell maturation in the thymus, mice show a significant reduction in absolute numbers of CD3+, CD4+ and CD8+ cells in spleen

• naive T cells released from the thymus are not able to proliferate to maintain naive T cell pool in periphery

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD4+ cells in spleen

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD8+ cells in spleen

endocrine/exocrine glands

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

cellular

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2