Phenotypes Associated with This Genotype

Genotype
MGI:6505535

• Allelic Composition: Med19^{tm1c(EUCOMM)Wtsi}/Med19^{tm1c(EUCOMM)Wtsi}; Tg(Adipoq-cre)1Evdr/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * FVB/NJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body fat mass ( J:298822 )

• reduced fat mass in both sexes at 12 weeks of age, as revealed by body composition analysis (EchoMRI)

increased lean body mass ( J:298822 )

• slightly increased lean mass in both sexes at 12 weeks of age, as revealed by EchoMRI

increased body weight ( J:298822 )

• increased body weight in both sexes at 9 and 12 weeks of age, but not at 3 or 6 weeks of age

• however, food intake is not significantly altered

enlarged liver ( J:298822 )

• enlarged liver at 12 weeks of age

increased liver weight ( J:298822 )

• markedly increased liver weight at 12 weeks of age

adipose tissue

decreased body fat mass ( J:298822 )

• reduced fat mass in both sexes at 12 weeks of age, as revealed by body composition analysis (EchoMRI)

whitened brown adipose tissue morphology ( J:298822 )

• brown adipose tissue (BAT) contains many white-adipocyte-like unilocular cells, indicating whitening of brown fat

• however, no change in brown adipose tissue (BAT) mass at 3 or 12 weeks of age

decreased gonadal fat pad weight ( J:298822 )

• severely reduced gonadal white fat depot (gWAT) weight in males as early as 3 weeks of age

decreased inguinal fat pad weight ( J:298822 )

• severely reduced inguinal white fat depot (iWAT) weight in males as early as 3 weeks of age

decreased mesenteric fat pad weight ( J:298822 )

• reduction in mesenteric WAT (mWAT) weight at 12 weeks of age

decreased retroperitoneal fat pad weight ( J:298822 )

• reduction in retroperitoneal WAT (rWAT) weight at 12 weeks of age

abnormal interscapular fat pad morphology ( J:298822 )

• interscapular BAT is much paler at 12 weeks of age, resembling WAT

abnormal white adipose tissue morphology ( J:298822 )

• small amount of residual WAT appears unhealthy with sparse, irregularly sized adipocytes among a fibrous extracellular matrix

decreased subcutaneous adipose tissue amount ( J:298822 )

• reduction in anterior subcutaneous WAT (asWAT) weight at 12 weeks of age

decreased white fat cell number ( J:298822 )

• sparse white adipocytes

abnormal white fat cell size ( J:298822 )

• irregularly sized white adipocytes

decreased white adipose tissue mass ( J:298822 )

• dramatic reduction in white adipose tissue mass at 12 weeks of age

• however, no change in brown adipose tissue (BAT) mass at 3 or 12 weeks of age

abnormal brown adipose tissue physiology ( J:298822 )

• mRNA expression of BAT genes, including Ucp1, is reduced

abnormal white adipose tissue physiology ( J:298822 )

• gene expression analysis of gWAT and iWAT revealed reduced mRNA levels of the adipocyte genes aP2, C/EBPalpha, and PPARgamma; mRNA expression of C/EBPbeta and KLF5 is either unchanged or elevated

homeostasis/metabolism

hyperglycemia ( J:298822 )

• both sexes are hyperglycemic

increased circulating insulin level ( J:298822 )

• markedly increased fasting serum insulin levels

impaired glucose tolerance ( J:298822 )

• both sexes are glucose intolerant

insulin resistance ( J:298822 )

• impaired response to insulin in insulin tolerance test

increased liver triglyceride level ( J:298822 )

• 4-fold increase in liver triglyceride content

liver/biliary system

enlarged liver ( J:298822 )

• enlarged liver at 12 weeks of age

increased liver weight ( J:298822 )

• markedly increased liver weight at 12 weeks of age

increased liver triglyceride level ( J:298822 )

• 4-fold increase in liver triglyceride content

hepatic steatosis ( J:298822 )

• massive accumulation of lipid droplets in liver at 12 weeks of age

pale liver ( J:298822 )

• pale liver at 12 weeks of age

abnormal liver physiology ( J:298822 )

• impaired hepatic insulin signaling, as assessed by phosphorylation of Akt in response to insulin

integument

decreased subcutaneous adipose tissue amount ( J:298822 )

• reduction in anterior subcutaneous WAT (asWAT) weight at 12 weeks of age