Phenotypes for Abhd6 MGI:6276000 MGI Mouse

abnormal pancreatic beta cell physiology ( J:215257 )

• islets show elevated monoacylglycerol production in response to glucose

• islet extracts show an approximate 50% reduced monoacylglycerol hydrolytic activity that is insensitive to inhibition by WWL70 unlike wild-type islets

decreased insulin secretion ( J:234876 )

• females on a chow diet show reduced insulin secretion during oral glucose tolerance test (OGTT)

increased insulin secretion ( J:215257 , J:234876 )

	• islets show an increase in glucose-stimulated insulin secretion at 8.3 and 16.7 mM glucose (J:215257) • islets show elevated KCl-stimulated insulin secretion (J:215257)
	• 6 week old males on a chow diet show no differences in glucose tolerance during oral glucose tolerance test despite increased insulin secretion (J:234876)

abnormal adipose tissue morphology ( J:234876 )

• visceral fat weight is reduced by about 40% in females, with no change in males, when fed a high fat diet

decreased body fat mass ( J:234876 )

• fat mass is markedly decreased in females and modestly decreased in males fed a high fat diet

decreased brown fat cell size ( J:234876 )

• mice fed a high fat diet show smaller adipocytes with fewer lipid deposits in the brown adipose tissue

decreased white fat cell size ( J:234876 )

• mice fed a high fat diet show smaller adipocytes in the visceral and inguinal adipose tissue

browned white adipose tissue morphology ( J:234876 )

• marker analysis indicates induction of browning-related genes in visceral and subcutaneous adipose tissue of high fat diet fed mice

increased adipocyte glucose uptake ( J:234876 )

• visceral fat from high fat diet fed mice shows enhanced glucose uptake under basal and insulin-stimulated conditions, with effects more pronounced in females than males

increased fatty acid beta-oxidation ( J:234876 )

• brown adipose tissue from high fat fed mice shows a nearly 2-fold increase in beta-oxidation

• males treated with the PPARalpha antagonist GW6471 show normalized beta-oxidation levels in brown adipose tissue

decreased insulin secretion ( J:234876 )

• females on a chow diet show reduced insulin secretion during oral glucose tolerance test (OGTT)

increased insulin secretion ( J:215257 , J:234876 )

	• islets show an increase in glucose-stimulated insulin secretion at 8.3 and 16.7 mM glucose (J:215257) • islets show elevated KCl-stimulated insulin secretion (J:215257)
	• 6 week old males on a chow diet show no differences in glucose tolerance during oral glucose tolerance test despite increased insulin secretion (J:234876)

decreased circulating insulin level ( J:234876 )

• mice fed a 60% high fat diet show much lower insulinemia during OGTT than controls

increased circulating insulin level ( J:215257 )

• plasma insulin during oral glucose tolerance test increases indicating elevated insulinemia with unchanged glycemia upon glucose load

• however, mice exhibit unaltered glucose tolerance and insulin sensitivity

abnormal body temperature homeostasis ( J:234876 )

• mice maintain their body temperature in cold better than controls during cold-induced thermogenesis for 3 hours

increased energy expenditure ( J:234876 )

• energy expenditure as a function of metabolic mass is higher in both the dark and light phases, during the last 24 hour period, and at both room temperature and 30 degrees Celsius in mice fed a high fat diet

• males treated with the PPARalpha antagonist GW6471 show reduced energy expenditure at 30 degrees

decreased susceptibility to diet-induced obesity ( J:234876 )

• mice show less body weight gain during high fat diet feeding

• the decrease in body weight gain is greater in females (about 30% at week 8) than in males (about 12% at week 8)

• body weight gain is still lower after pair feeding for 2 weeks

• males treated with the PPARalpha antagonist GW6471 show slightly elevated body weight gain without altering food intake

increased oxygen consumption ( J:234876 )

• mice fed a high fat diet exhibit elevated volume of oxygen during light and dark phases and during the last 24 hour period at both room temperature and 30 degrees Celsius, indicating elevated respiration

• however, respiratory exchange ratio is not affected

• males treated with the PPARalpha antagonist GW6471 show lowered oxygen consumption

improved glucose tolerance ( J:234876 )

• mice fed a 60% high fat diet for 8 weeks show improved glucose tolerance during oral glucose tolerance test (OGTT)

• females on a chow diet show better glucose tolerance than wild-type mice, however males show normal glucose metabolism on regular chow

• glucose tolerance remains improved after pair feeding

increased insulin sensitivity ( J:234876 )

• glucose infusion rate (index of insulin sensitivity) in high-fat diet fed mice is higher

• mice fed a 60% high fat diet show enhanced insulin sensitivity during insulin tolerance test

• females on a chow diet show slightly reduced body weight gain over 3-24 weeks of age and cumulative food intake over 5-16 weeks of age and show better glucose tolerance than wild-type mice but with reduced insulin secretion during OGTT, indicating that females are more insulin sensitive

• proteins related to insulin resistance such as ICAM-1, IGFBP-1, and resistin are decreased in the plasma

• insulin sensitivity remains increased after pair feeding

• males treated with the PPARalpha antagonist GW6471 show lowered insulin sensitivity

abnormal lipid level ( J:215257 )

• islets exhibit elevated total and 1-monoacylglycerol content and a moderate increase in 2-monoacylglycerol at high glucose

• high glucose induces a higher increase in 1-palmitoylglycerol and 1-stearoylglycerol in islets

• maximal changes in islets are seen in saturated-fatty acid-containing monoacylglycerol species

decreased liver triglyceride level ( J:234876 )

• liver triglyceride content is decreased in mice fed a high fat diet

abnormal enzyme/coenzyme activity ( J:234876 )

• total 1-MAG hydrolase activity is decreased in visceral and brown adipose tissues of high fat diet fed mice

decreased food intake ( J:234876 )

• mice show reduced cumulative food intake during high fat diet feeding showing an approximate 7% reduction at weeks 6-8 of the diet

• females on a chow diet show slightly reduced cumulative food intake over 5-16 weeks of age

• food intake is unaltered in males treated with the PPARalpha antagonist GW6471

increased locomotor activity ( J:234876 )

• mice fed a high fat diet show increased locomotor activity over a 24 hour time period and during the dark phase, with a greater increase in females than males

• however, mice do not exhibit changes in anxiety or depressive behavior in the elevated-plus maze, the open-field, and forced swim test

• males treated with the PPARalpha antagonist GW6471 show reduced locomotor activity

increased adipocyte glucose uptake ( J:234876 )

• visceral fat from high fat diet fed mice shows enhanced glucose uptake under basal and insulin-stimulated conditions, with effects more pronounced in females than males

increased muscle cell glucose uptake ( J:234876 )

• soleus muscle from high fat diet fed mice shows enhanced glucose uptake under basal and insulin-stimulated conditions, with effects more pronounced in females than males