Phenotypes Associated with This Genotype

Genotype
MGI:6259577

• Allelic Composition: Cdk4^tm1.1Bbd/Cdk4⁺; Tg(Mt1-Hgf)#Lmb/0
• Genetic Background: B6.Cg-Cdk4^tm1.1Bbd Tg(Mt1-Hgf)#Lmb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased incidence of tumors by chemical induction ( J:111992 )

• in a 2-step (DMBA/TPA) skin carcinogenesis protocol, all (15 of 15) mice develop progressively growing melanomas and have to be sacrificed between 14 and 16 weeks of age with an average of 32 cutaneous melanomas

• DMBA/TPA-induced melanomas involve the epidermo-dermal junction and show vertical invasive growth in the underlying dermis

• only 3 of 15 DMBA/TPA-treated mice develop a single papilloma in skin

increased metastatic potential ( J:111992 )

• DMBA/TPA-treated mice show locoregional metastatic growth of melanoma cells in draining lymph nodes as well as numerous melanoma micrometastases in lung tissue

• in lymph nodes of DMBA/TPA-treated mice, melanoma cells are mostly spindle-shaped cells with little/no melanin that eventually disrupt lymph node architecture and displace lymphoid cells; however, heavily pigmented epitheloid melanoma cells are also observed

• in DMBA/TPA-treated mice, the average number of lung metastases is comparable to that in Tg(Mt1-HGFSF)#Lmb/0 mice

decreased tumor growth/size ( J:111992 )

• DMBA/TPA-induced melanomas grow with a slight delay relative to those in Cdk4^tm1.1Bbd/Cdk4^tm1.1Bbd Tg(Mt1-HGFSF)#Lmb/0 mice

immune system

abnormal axillary lymph node morphology ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show heavily pigmented axillary lymph nodes

enlarged axillary lymph nodes ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show enlarged axillary lymph nodes due to growth of melanoma cells

abnormal inguinal lymph node morphology ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show heavily pigmented inguinal lymph nodes

enlarged inguinal lymph nodes ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show enlarged inguinal lymph nodes due to growth of melanoma cells

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:111992 )

• in a 2-step (DMBA/TPA) skin carcinogenesis protocol, all (15 of 15) mice develop progressively growing melanomas and have to be sacrificed between 14 and 16 weeks of age with an average of 32 cutaneous melanomas

• DMBA/TPA-induced melanomas involve the epidermo-dermal junction and show vertical invasive growth in the underlying dermis

• only 3 of 15 DMBA/TPA-treated mice develop a single papilloma in skin