Phenotypes Associated with This Genotype

Genotype
MGI:6259550

• Allelic Composition: Cdk4⁺/Cdk4⁺; Tg(Mt1-Hgf)#Lmb/0
• Genetic Background: B6.Cg-Cdk4^tm1.1Bbd Tg(Mt1-Hgf)#Lmb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased incidence of tumors by chemical induction ( J:111992 )

• in a 2-step (DMBA/TPA) skin carcinogenesis protocol, all (18 of 18) mice develop slowly growing skin melanomas with an average of 12 cutaneous melanomas

• DMBA/TPA-induced melanomas involve the epidermo-dermal junction and show vertical invasive growth into the dermis; however, no proliferative activity of melanoma cells is detected by Ki67 staining

• 10 of 18 DMBA/TPA-treated mice also develop large subcutaneous cystic tumors filled with serous liquid and have to be sacrificed by 30 weeks of age

• all DMBA/TPA-treated mice develop on average 3 papillomas in the skin; these appear early after carcinogen treatment

increased skin nevi incidence ( J:111992 )

• at 30 weeks of age, untreated mice exhibit melanocytic nevi in skin

• however, untreated mice are all alive and healthy at 1 year of age

increased metastatic potential ( J:111992 )

• DMBA/TPA-treated mice show locoregional metastatic growth of melanoma cells in draining lymph nodes as well as numerous melanoma micrometastases in lung tissue

• in lymph nodes of DMBA/TPA-treated mice, melanoma cells are mainly spindle-shaped cells with little/no melanin that eventually disrupt lymph node architecture and displace lymphoid cells; however, heavily pigmented epitheloid melanoma cells are also observed

• in DMBA/TPA-treated mice, the average number of lung metastases is comparable to that in Cdk4^tm1.1Bbd/Cdk4⁺ Tg(Mt1-HGFSF)#Lmb/0 mice

integument

increased skin nevi incidence ( J:111992 )

• at 30 weeks of age, untreated mice exhibit melanocytic nevi in skin

• however, untreated mice are all alive and healthy at 1 year of age

immune system

abnormal axillary lymph node morphology ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show heavily pigmented axillar lymph nodes

abnormal inguinal lymph node morphology ( J:111992 )

• at sacrifice, DMBA/TPA-treated mice show heavily pigmented inguinal lymph nodes

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:111992 )

• in a 2-step (DMBA/TPA) skin carcinogenesis protocol, all (18 of 18) mice develop slowly growing skin melanomas with an average of 12 cutaneous melanomas

• DMBA/TPA-induced melanomas involve the epidermo-dermal junction and show vertical invasive growth into the dermis; however, no proliferative activity of melanoma cells is detected by Ki67 staining

• 10 of 18 DMBA/TPA-treated mice also develop large subcutaneous cystic tumors filled with serous liquid and have to be sacrificed by 30 weeks of age

• all DMBA/TPA-treated mice develop on average 3 papillomas in the skin; these appear early after carcinogen treatment