Phenotypes Associated with This Genotype

Genotype
MGI:6110004

• Allelic Composition: Plcz1^em2Jparr/Plcz1^em2Jparr
• Genetic Background: C57BL/6-Plcz1^em2Jparr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:243414 )

♂N • normal viability of epididymal sperm

♂N • normal sperm capacitation

♂N • normal acrosome reaction in response to progesterone or ionomycin

♂N • normal hyperactivated motility of epididymal sperm

♂N • normal motility of epididymal sperm

reduced male fertility ( J:243414 )

♂ • average 2.3 live pups (vs 12.7 WT) per female partner

♂ • average 3.2 live pups (vs 7.8 WT) per male

abnormal egg activation ( J:243414 )

• no induction of Ca2+ oscillations in oocytes after intracytoplasmic sperm injection or in vitro fertilization

polyspermy ( J:243414 )

• increase in polyspermy (19/82 vs 3/37 WT) of fertilized eggs 6 h after IVF

impaired fertilization ( J:243414 )

• no induction of Ca2+ oscillations in oocytes after intracytoplasmic sperm injection or in vitro fertilization

• very few eggs reaching two-cell (1/47 vs 35/40 WT) and none blastocyst (0/47 vs 27/40 WT) stage after intracytoplasmic sperm injection

• very few eggs with two pronuclei 25 h after hCG treatment and in vivo fertilization (1/43 vs 19/25 WT after 22 h)

• very few eggs in two-cell stage 38 h after hCG treatment and in vivo fertilization (9/43 vs 18/25 WT after 32 h)