Phenotypes Associated with This Genotype

Genotype
MGI:5824823

• Allelic Composition: Tg(Prox1-EGFP)KY221Gsat/0
• Genetic Background: involves: FVB/NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Galectin-8 markedly enhances VEGF-C-induced lymphangiogenesis in vivo in Tg(Prox1-EGFP)KY221Gsat/0 mice

immune system

abnormal lymphangiogenesis ( J:235778 )

• in corneal micropocket assays, corneas implanted with both VEGF-C and galectin-8 pellets show enhanced VEGF-C induced lymphangiogenesis relative to corneas implanted with either VEGF-C or galectin-8 pellets alone, as calculated by the lymphatic vessel area on day 7 post-surgery

• after suture placement or silver nitrate cauterization to induce corneal inflammation, treatment with TDG (a pan inhibitor of galectins) or Gal-8N (a dominant negative inhibitor of galectin-8) significantly suppresses both suture- and cautery-induced inflammatory lymphangiogenesis, as quantified by the lymphatic vessel area at the end of the treatment period

abnormal lymphatic vessel endothelial cell morphology ( J:235778 )

• in corneal micropocket assays, corneas implanted with either VEGF-C or galectin-8 pellets exhibit >35% of proliferative lymphatic endothelial cells (Prox1+ Ki67+ LECs) in lymphangiogenic areas, whereas corneas implanted with control pellets show <4% of Prox1+ Ki67+ LECs on day 7 post-surgery