Phenotypes Associated with This Genotype

Genotype
MGI:5810004

• Allelic Composition: Cep120^tm1.2Blnw/Cep120^tm1.2Blnw
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Developmentof Cep120^tm1.2Blnw/Cep120^tm1.2Blnw embryos is delayed and the heart loops in the opposite direction

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:220629 )

• homozygotes die around E9.5

embryo

abnormal developmental patterning ( J:220629 )

• homozygous embryos fail to bend

embryonic growth arrest ( J:220629 )

• homozygotes exhibit developmental arrest at E8.5

cardiovascular system

abnormal direction of heart looping ( J:220629 )

• E9.5 homozygotes display heart looping in the opposite direction

cellular

abnormal cell morphology ( J:220629 )

• most primary MEFs lack either one or both centrioles, whereas the vast majority of wild-type MEFs each contain two centrioles, as indicated by gamma-tubulin staining

• immunostaining for Cep164, 2700049A03Rik (Ta3), and Odf2 indicates that the missing centriole is the mother centriole; thus, the remaining single centriole is the daughter centriole

abnormal cilium morphology ( J:220629 )

• primary MEFs derived from E9.5 mutant embryos lack cilia, as shown by the absence of staining for acetylated tubulin and Arl13b, two cilia markers