Phenotypes Associated with This Genotype

Genotype
MGI:5807457

• Allelic Composition: Izumo1r^{tm1a(KOMP)Wtsi}/Izumo1r^{tm1a(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6N * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

female infertility ( J:209568 )

♀ • female homozygotes fail to produce any litters during 3 months of continuous mating with fertile wild-type males

♀ • however, mating behavior is normal and female homozygotes respond to hormone treatment by ovulating morphologically normal eggs at close to wild-type numbers

impaired fertilization ( J:209568 )

• mutant eggs recovered at E0.5 after superovulation and natural mating are not fertilized and show more sperm within their perivitelline space relative to wild-type eggs

• zona-free mutant eggs cannot be fertilized with wild-type sperm in IVF assays, as determined by the absence of fused sperm and formed pronuclei

• in vitro, acrosome-reacted (GFP-negative) normal sperm identified using Tg(Acr-EGFP)1Osb reporter mice bind less efficiently to zona-free mutant eggs than wild-type control eggs