Analysis Tools
mortality/aging
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• homozyous mutant mice die between E13.5 and E15.5
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embryo
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• average embryo sizes and placental weights in homozygous mutant fetuses were comparable to those of control siblings
• organization of the placental junctional zone, including primary trophoblast giant cell and spongiotrophoblast layers in homozygous null mutant placentae, was similar to controls
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• an anomalous labyrinth layer was noted in E13.5 and E15.5 placentae; abnormalities were indistinguishable at E10.5 but very pronounced at E15.5 dpc
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• the maternal and fetal vessel spaces in the labyrinth of mutant mice were taken up by large densely packed cell aggregates
• both maternal and fetal vascular spaces were dramatically reduced, while the labyrinth nonvessel area was markedly increased
• the labyrinth of mutant E15.5 placentae displayed an increase in the number of cells undergoing division, especially the cells in the densely packed cell aggregates; TUNEL assays did not exhibit a significant difference between Wt and mutant placentae so apoptosis was unaffected
• cell clusters likely were aggregates of trophoblast stem cells and trophoblast progenitors based on expression analysis
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integument
cardiovascular system
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• the maternal and fetal vessel spaces in the labyrinth of mutant mice were taken up by large densely packed cell aggregates
• both maternal and fetal vascular spaces were dramatically reduced, while the labyrinth nonvessel area was markedly increased
• the labyrinth of mutant E15.5 placentae displayed an increase in the number of cells undergoing division, especially the cells in the densely packed cell aggregates; TUNEL assays did not exhibit a significant difference between Wt and mutant placentae so apoptosis was unaffected
• cell clusters likely were aggregates of trophoblast stem cells and trophoblast progenitors based on expression analysis
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