Analysis Tools
behavior/neurological
 N |
• despite a trend toward decreased immobility time in the tail suspension test (TST), male homozygotes show no significant changes in the time spent curling and swinging in the TST, or in the duration of immobility, climbing, and swimming in the forced swim test, suggesting that depression-related behavior is not significantly altered
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• in the elevated plus maze test, male homozygotes spend significantly less time on the open arms and more time on the closed arms of the maze relative to wild-type controls; however, neither the total distance traveled nor the total number of entries into any arm is significantly altered during the 5-min test session
• in the light/dark box test, the time spent and the distance traveled in the light compartment are significantly reduced relative to wild-type controls; however, locomotion expressed as the total distance traveled is not significantly altered during the 10-min test session
• in the open field test, homozygotes spend significantly less time in the central area of the field, visit the central area significantly less often, and shed a significantly higher number of fecal boli than wild-type controls
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• in the open field test, male homozygotes show a significant reduction in total traveling distance relative to wild-type controls
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• in a social interaction test, male homozygotes do not show a significant preference for the compartment containing a stranger mouse, neither in terms of time spent in the compartment or in the immediate vicinity nor in terms of visits to the compartment or to the immediate vicinity of the stranger mouse, unlike wild-type controls
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endocrine/exocrine glands
 N |
• despite their anxiogenic-like phenotype, homozygotes show normal sudomotor responses with no significant differences in the total number of secreting sweat glands in the hind paw at room temperature (basal) or after pilocarpine injection, thermal stimulation, and immobilization (stress) relative to wild-type controls
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nervous system
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• homozygotes display normal brain development, as shown by NeuN, GABA, and calcium/calmodulin kinase II immunoreactivities in the hippocampus and the basolateral amygdala
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homeostasis/metabolism
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N |
• unlike results reported by the EMMA network in J:82809, plasma cholesterol and triglyceride levels of 14- to 15-wk-old male homozygotes are within the range of baseline levels reported for C57BL/6 mice
• normal amino acid profiles in serum, whole brain, and liver tissue in male homozygotes relative to wild-type males
• normal expression levels of all examined serotonin (5-HT)-related genes
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hematopoietic system
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N |
• male homozygotes exhibit normal clinical hematology parameters, as shown by the status of blood cell components, including erythrocytes, leukocytes, and thrombocytes
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