Phenotypes Associated with This Genotype

Genotype
MGI:5688361

• Allelic Composition: Vgf^tm1Vlcg/Vgf^tm1Vlcg
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal adrenal medulla morphology ( J:212045 )

• at 6-8 weeks of age, Western blot analysis revealed that chromogranin B (CgB) protein levels in the adrenal medulla are reduced to 47.3% of wild-type levels

• in contrast, adrenal chromogranin A, and secretogranin II and III levels are not significantly altered

abnormal noradrenergic chromaffin cell morphology ( J:212045 )

• at 6-8 weeks of age, the mean size of norepinephrine-containing large dense core vesicles (LDCVs) is reduced relative to wild-type adrenal chromaffin cells, and the relative number of smaller LDCVs is increased

• however, the mean number of norepinephrine-containing LDCVs per square micrometer of cytoplasm in adrenal chromaffin cells is unaffected

homeostasis/metabolism

abnormal adrenaline level ( J:212045 )

• epinephrine content is significantly increased in mutant adrenal glands relative to wild-type controls

• in contrast, cardiac muscle epinephrine levels are not significantly elevated

increased circulating adrenaline level ( J:212045 )

• plasma epinephrine levels are significantly increased relative to wild-type controls

increased noradrenaline level ( J:212045 )

• norepinephrine content is significantly increased in mutant adrenal glands relative to wild-type controls

• in contrast, plasma and cardiac muscle norepinephrine levels are not significantly elevated

nervous system

abnormal noradrenergic chromaffin cell morphology ( J:212045 )

• at 6-8 weeks of age, the mean size of norepinephrine-containing large dense core vesicles (LDCVs) is reduced relative to wild-type adrenal chromaffin cells, and the relative number of smaller LDCVs is increased

• however, the mean number of norepinephrine-containing LDCVs per square micrometer of cytoplasm in adrenal chromaffin cells is unaffected

cardiovascular system

hypertension ( J:212045 )

• homozygotes are hypertensive

increased mean systemic arterial blood pressure ( J:212045 )

• homozygotes display a significant increase in MAP relative to wild-type controls

• however, the heart rate is not significantly altered

increased systemic arterial diastolic blood pressure ( J:212045 )

• homozygotes display a significant increase in diastolic blood pressure (DBP) relative to wild-type controls

increased systemic arterial systolic blood pressure ( J:212045 )

• homozygotes display a significant increase in systolic blood pressure (SBP) relative to wild-type controls