Analysis Tools
embryo
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• embryos isolated at the 8 cell stage then treated with 4-hydroxytamoxifen (4-OHT) for 24 hours then examined 24 hours later show impaired formation of the primitive endoderm
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• impaired lineage segregation of the ICM in 4-OHT treated cultured embryos
• after 5 days in culture 4-OHT treated embryos produce sheets of trophoblast giant cells with no discernible ICM or primitive endoderm derivatives
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