Phenotypes for Prdm16 Tg(Adipoq-cre)1Evdr MGI:5564786 MGI Mouse

abnormal adipose tissue morphology ( J:208683 )

• subcutaneous adipose tissue contains larger adipocytes and fewer smaller multiocular UCP1+ adipocytes relative to control mice following cold exposure

• subcutaneous adipose tissue from mice following 6 weeks on high fat diet has increased numbers of crown like structures, however, there are no changes in visceral (abdominal) fat

• subcutaneous adipose tisse from mice following 6 weeks on high fat diet has increased numbers of CD11b+F4/80+ macrophages

increased subcutaneous adipose tissue amount ( J:208683 )

• subcutaneous fat mass from male mice after 16 weeks on high fat diet is increased to twice that of controls, epididymal and brown adipose tissue are unchanged

increased total body fat amount ( J:208683 )

• body composition analysis after 16 weeks on high fat diet indicates increased fat mass with no change in lean body mass

abnormal adipose tissue distribution ( J:208683 )

• subcutaneous fat mass is doubled as compared to controls after 16 weeks on high fat diet; epididymal and BAT masses are unchanged

increased fat cell size ( J:208683 )

• subcutaneous adipocytes from male mice after 18 weeks on high fat diet exhibit a 33% increase in mean adipocyte area

abnormal adipose tissue physiology ( J:208683 )

• O₂ consumption is reduced in subcutaneous adipose pads, but not in brown adipose pads, at baseline (36% reduced) and following stimulation with a beta-adrenergic agonist (49% reduced)

decreased adipocyte glucose uptake ( J:208683 )

• glucose uptake is reduced in subcutaneous fat (79% decrease) and visceral fat (53% decrease) after 6 weeks on high fat diet as compared to controls

abnormal brown adipose tissue thermogenesis ( J:208683 )

• subcutaneous adipocytes exhibit a blunted response to stimuli such as isoproterenol that induce a thermogenic gene program (beige adipocytes)

increased susceptibility to weight gain ( J:208683 )

• mice exhibit increased weight gain after 16 weeks on high-fat, high-carbohydrate diet relative to control mice

increased macrophage cell number ( J:208683 )

• subcutaneous adipose tissue from mice following 6 weeks on high fat diet has increased numbers of CD11b+F4/80+ macrophages, but macrophage numbers in visceral adipose tissue and spleen are unchanged

increased circulating insulin level ( J:208683 )

• increased fasting plasma insulin levels (55%) in mice after 6 weeks on high fat diet

abnormal oxygen consumption ( J:208683 )

• unlike controls, mutant mice do not exhibit increased O₂ consumption following stimulation with a beta-adrenergic agonist

increased respiratory quotient ( J:208683 )

• mice exhibit an increased respiratory exchange ratio (RER), but no increase in O₂ consumption

insulin resistance ( J:208683 )

• a reduced glucose infusion rate (64% of controls) is observed in mice after 6 weeks on high fat diet

• decreased whole body glucose uptake is observed in mice after 6 weeks on high fat diet

• clamped insulin levels do not increase as much as controls in mice after 6 weeks on high fat diet

increased liver triglyceride level ( J:208683 )

• increased liver tryglycerides are observed in mice after 6 weeks on high fat diet as compared to controls