Mouse Genome Informatics
either: (involves: 129 * 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6)
phenotype observed in females
phenotype observed in males
N normal phenotype
adipose tissue
• marker analysis indicates abnormal brown adipose tissue (BAT) development
• BAT cells contain large unilocular fat droplets compared to multilocular fat droplets in wild-type BAT
• preadiopocytes do not differentiate

• preadiopocytes do not differentiate
• mitochondria of BAT and skeletal muscle, but not in the heart, shows disorganized cristae
• oxygen consumption is decreased in mitochondria when pyruvate and malate are used as substrates for complex I activity and succinate for complex II activity
• addition of GDP decreases oxygen consumption in wild-type but not mutant mitochondria

• increase in body weight is seen as early as 12-16 weeks of age on a normal chow diet
• by 12 months of age, mutants are about 20 grams heavier than wild-type mice
• however, mutants exhibit normal food intake and physical activity

• body temperatures are lower in mutants than controls when exposed to 4 degrees C for 12 hours
• produce 20% less heat during both day and night cycles
• abnormal glucose clearance suggestive of insulin resistance

Mouse Models of Human Disease
Obesity 601665 J:195099