Analysis Tools
hematopoietic system
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• bone marrow cells transformed with a MLL-AF9 retrovirus then a cre-expressing retrovirus produce decreased numbers of smaller blast-like colonies on methylcellulose compared with control cells
• however, myeloid progenitor assays are normal in cells not transformed with MLL-AF9 or transformed with HoxA9/Meis1a
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• bone marrow cells transformed with MLL-AF9-expressing retrovirus, transduced with a cre-expressing retrovirus and transplanted into irradiated recipients fail to cause leukemia unlike control cells
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neoplasm
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• transplanted MLL-AF9 leukemia cells transduced with a cre-expressing vector form fewer, smaller and less compact colony composed of terminally differentiated cells with reduced cell proliferation compared with control cells
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