Phenotypes for Notch1 Tg(Wt1-cre)#Jbeb MGI:5316086 MGI Mouse

embryonic lethality during organogenesis ( J:178290 )

• embryos start to die shortly after E13.5

thin myocardium compact layer ( J:178290 )

• compact myocardium thickness is signficantly reduced in mutants

• number of nuclei is lower (25-30%) at E13.5, with reduction in proliferating cells observed compared to control

myocardium hypoplasia ( J:178290 )

• reduction in number of smooth muscle cells in the compact myocardium is observed at E13.5

abnormal coronary vessel morphology ( J:178290 )

• at E13.5 hearts exhibit severe reduction and disorganization of the coronary vascular plexus

• abnormal development of CVs is associated with endocardial-subepicardial connections that result in cysts with endothelial lining connected to the outer ventricular myocardium

absent coronary vessels ( J:178290 )

• coronary arteries are absent from the compact vemtricular myocardium, specifically the deep myocardium; coronary veins occupy a larger area of the subepicardium

hemorrhage ( J:178290 )

• at E13.5 most embryos display body hemorrhages and die shortly afterwards

hemopericardium ( J:178290 )

• at E13.5 most embryos display pericardial hemorrhages and die shortly afterwards