Phenotypes Associated with This Genotype

Genotype
MGI:5285848

• Allelic Composition: Tsix^tm1.1Awu/Tsix⁺; Tg(CAG-EGFP)50Osb/0; Gt(ROSA)26Sor^tm1(rtTA)Awu/Gt(ROSA)26Sor^tm1(rtTA)Awu
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C3H/HeSlc * C57BL/6J * C57BL/6Slc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

embryonic growth retardation ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 exhibit severe developmental defect at E7.5 compared with control mice

♀ • however, no developmental defect is observed when the allele is maternally inherited or when doxycycline treated blastocysts with paternally inherited Tsix^tm1.1Awu are transplanted into wild-type recipients

abnormal placenta labyrinth morphology ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited maternally, female mice treated with doxycycline from E6.5 exhibit reduced density of fetal capillaries in the placental labyrinth compared with control mice

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in fetal capillaries compared with control mice

absent placental labyrinth ( J:174672 )

♀ • in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

small placenta ( J:174672 )

♀ • at E13.5 in female mice treated with doxycycline from E6.5 or E7.5 when Tsix^tm1.1Awu is inherited paternally

♀ • mild at E13.5 in female mice treated with doxycycline from E8.5 when Tsix^tm1.1Awu is inherited paternally

♀ • however, female mice treated with doxycycline from E9.5 exhibit normal placenta size at E13.5 when the allele is inherited paternally

abnormal trophoblast layer morphology ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in all trophoblast cell types compared with control mice

abnormal trophoblast giant cell morphology ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit overabundant and massively enlarged compared to in control mice

increased trophoblast giant cell number ( J:174672 )

♀ • in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

absent spongiotrophoblast layer ( J:174672 )

♀ • absent in severely affected female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

cellular

abnormal DNA methylation ( J:174672 )

♀ • increased in female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

abnormal dosage compensation, by inactivation of X chromosome ( J:174672 )

♀ • in female mice treated with doxycycline from E6.5 when Tsix^tm1.1Awu is inherited paternally

abnormal imprinting ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 fail to imprint and inactivated the paternal X chromosome unlike in control mice

cardiovascular system

abnormal capillary morphology ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited maternally, female mice treated with doxycycline from E6.5 exhibit reduced density of fetal capillaries in the placental labyrinth compared with control mice

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E6.5 exhibit a severe defect in fetal capillaries compared with control mice

decreased capillary density ( J:174672 )

♀

growth/size/body

embryonic growth retardation ( J:174672 )

♀ • when Tsix^tm1.1Awu is inherited paternally, female mice treated with doxycycline from E0.5 exhibit severe developmental defect at E7.5 compared with control mice

♀ • however, no developmental defect is observed when the allele is maternally inherited or when doxycycline treated blastocysts with paternally inherited Tsix^tm1.1Awu are transplanted into wild-type recipients