Phenotypes for Edil3 Rac1 MGI:5050115 MGI Mouse

prenatal lethality, complete penetrance ( J:166095 )

• survive longer than germ line null mice

• survive until E8.5

increased embryonic tissue cell apoptosis ( J:173526 )

• in the mesoderm and basal regions of the epiblast at E7.5

• at E6.5 and E7.5, cell death is increased in the anterior half of the embryo compared to the posterior half

abnormal developmental patterning ( J:173526 )

• expression analysis indicates organization of midline structures is abnormal

• the midline is broad at E8.5

abnormal mesoderm development ( J:173526 )

• mesoderm cells are specified but migration away from the primitive streak is impaired

• mesodermal cells are round, with just a few short protrusions

decreased embryo size ( J:173526 )

• the embryonic region is smaller compared to control littermates from E7.5 onwards

abnormal notochordal plate morphology ( J:173526 )

• expression analysis indicates the notochordal plate is wider and shorter at E7.75

abnormal neural tube closure ( J:173526 )

• the neural plate is present but fails to initiate neural tube closure

abnormal primitive streak morphology ( J:173526 )

• during epithelial to mesenchymal transition in the primitive streak breakdown of the basement membrane appears somewhat disrupted with large laminin aggregates seen on nascent mesodermal cells

• accumulation of T expressing cells in the primitive streak indicating an impairment in migration of cells away from the streak

abnormal primitive node morphology ( J:173526 )

• node cells are specified but fail to coalesce to form a normal node

impaired somite development ( J:173526 )

• properly organized somites do not form

decreased cell adhesion ( J:173526 )

• cultured cells from E7.5 embryos fail to adhere to either fibronectin or Matrigel matrices