Phenotypes Associated with This Genotype

Genotype
MGI:4942158

• Allelic Composition: Cebpa^tm1Dgt/Cebpa^tm1Dgt; Tg(SFTPC-rtTA)5Jaw/0; Tg(tetO-cre)1Jaw/0
• Genetic Background: involves: 129 * 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, incomplete penetrance ( J:105593 )

• although born in Mendelian ratios, most mice exposed to doxycycline from conception die of respiratory failure at birth

respiratory system

abnormal pulmonary alveolus morphology ( J:105593 )

• doxycycline-exposed newborn mice show a reduction of airspace, sometimes obliterating the alveolar structure

abnormal pulmonary alveolus epithelial cell morphology ( J:105593 )

• doxycycline-exposed newborn mice display increased numbers of Ki-67-positive alveolar cells, indicating increased alveolar cell proliferation

• in addition, neonatal alveolar cells show significantly decreased numbers of TUNEL-positive cells, indicating reduced apoptosis

absent type I pneumocytes ( J:105593 )

• doxycycline-exposed newborn mice lack type I alveolar cells

abnormal type II pneumocyte morphology ( J:105593 )

• alveoli of doxycycline-exposed newborn mice are lined by immature cuboidal type II cells with a clear cytosol, round nuclei and high glycogen content, indicating a type II cell differentiation arrest

absent alveolar lamellar bodies ( J:105593 )

• immature type II alveolar cells of doxycycline-exposed newborn mice lack lamellar bodies

absent type II pneumocytes ( J:105593 )

• doxycycline-exposed newborn mice lack mature type II alveolar cells

increased pulmonary alveolus wall thickness ( J:105593 )

• doxycycline-exposed newborn mice display thicker alveolar walls due to marked cellular accumulation

respiratory distress ( J:105593 )

• 86% of mice exposed to doxycycline from conception show signs of respiratory distress within hours after birth

respiratory failure ( J:105593 )

• most mice exposed to doxycycline from conception to birth display respiratory failure

abnormal surfactant secretion ( J:105593 )

• doxycycline-exposed newborn mice show a marked loss of surfactant protein B (SP-B) by immunostaining

• mRNAs for SP-A and SP-D are significantly down-regulated

liver/biliary system

liver/biliary system phenotype ( J:105593 )

N • doxycycline-exposed mice have a normal liver architecture and show normal Cebpa mRNA levels in liver

hematopoietic system

hematopoietic system phenotype ( J:105593 )

N • doxycycline-exposed mice exhibit normal granulocytic differentiation in fetal liver and contain granulocytes in peripheral blood