Phenotypes Associated with This Genotype

Genotype
MGI:4850097

• Allelic Composition: Pik3cd^tm2.1Tnr/Pik3cd^tm2.1Tnr; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased germinal center B cell number ( J:164283 )

• 7-fold following immunization with NP-KLH

• following immunization with SRBCs (sheep red blood cells)

decreased T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

decreased CD4-positive, alpha-beta T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

• slightly in B cell follicles

• significantly in PNA+ IgD- germinal center areas

decreased T follicular helper cell number ( J:164283 )

• following immunization with NP-KLH of SRBCs (sheep red blood cells)

decreased CD8-positive, alpha-beta T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

decreased spleen germinal center number ( J:164283 )

• following immunization with NP-KLH, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice

decreased spleen germinal center size ( J:164283 )

• following immunization with SRBCs (sheep red blood cells)

abnormal adaptive immunity ( J:164283 )

• following immunization and a later antigen boost, mice exhibit defective immunological memory compared with wild-type mice

abnormal humoral immune response ( J:164283 )

decreased IgG1 level ( J:164283 )

• following immunization with NP33-BSA, the NP-specific IgG1 titers are decreased compared to in similarly treated wild-type mice

• following immunization with NP3-BSA, high-affinity NP-specific IgG1 response is severely impaired compared to in similarly treated wild-type mice

• fewer IgG1-secreting antibody forming cells are detected in the spleen and bone marrow compared to in wild-type mice

hematopoietic system

decreased germinal center B cell number ( J:164283 )

• 7-fold following immunization with NP-KLH

• following immunization with SRBCs (sheep red blood cells)

decreased T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

decreased CD4-positive, alpha-beta T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

• slightly in B cell follicles

• significantly in PNA+ IgD- germinal center areas

decreased T follicular helper cell number ( J:164283 )

• following immunization with NP-KLH of SRBCs (sheep red blood cells)

decreased CD8-positive, alpha-beta T cell number ( J:164283 )

• modestly in the spleen without altering lymph node numbers

decreased spleen germinal center number ( J:164283 )

• following immunization with NP-KLH, the ratio of germinal centers per B cell follicle is reduced compared to in similarly treated wild-type mice

decreased spleen germinal center size ( J:164283 )

• following immunization with SRBCs (sheep red blood cells)

decreased IgG1 level ( J:164283 )

• following immunization with NP33-BSA, the NP-specific IgG1 titers are decreased compared to in similarly treated wild-type mice

• following immunization with NP3-BSA, high-affinity NP-specific IgG1 response is severely impaired compared to in similarly treated wild-type mice

• fewer IgG1-secreting antibody forming cells are detected in the spleen and bone marrow compared to in wild-type mice