Phenotypes Associated with This Genotype

Genotype
MGI:4836216

• Allelic Composition: Tg(Mcpt8-cre)1Voeh/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:164432 )

N • deletion of basophils does not affect populations of other leukocytes in mesenteric lymph nodes or spleen

decreased basophil cell number ( J:164432 )

• greater than 90% of basophils are spontaneously deleted; basophil development is not rescued by exogenous Il3 and it is suggested that basophils are sensitive to cre toxicity resulting from nonspecific recombination events of cryptic loxP sites in the genome

immune system

immune system phenotype ( J:164432 )

N • deletion of basophils does not affect populations of other leukocytes in mesenteric lymph nodes or spleen; no defects in T cell development or basal T cell activation are observed

N • response to induction of active systemic anaphylaxis appears normal

N • passive systemic IgE-mediated anaphylaxis appears normal; passive IgG1-mediated anaphylaxis can be induced in mutants and controls by administration of IgG1 antibodies

decreased basophil cell number ( J:164432 )

• greater than 90% of basophils are spontaneously deleted; basophil development is not rescued by exogenous Il3 and it is suggested that basophils are sensitive to cre toxicity resulting from nonspecific recombination events of cryptic loxP sites in the genome

decreased inflammatory response ( J:164432 )

• in contrast to control mice, IgE-mediated chronic inflammation of the skin (IgE-CAI) is abolished in transgenic mice

• animals reconstituted with in vitro-generated basophils have the IgE-CAI response restored