Phenotypes Associated with This Genotype

Genotype
MGI:4819582

• Allelic Composition: Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺; Lin9^tm1.1Sgau/Lin9^tm1.1Sgau
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:162678 )

• adult mice die 7 days after tamoxifen treatment

digestive/alimentary system

abnormal small intestinal crypt cell proliferation ( J:162678 )

• tamoxifen-treated mice exhibit reduced cell proliferation with abnormal nuclei and mitotic increased nuclear area compared with wild-type mice

abnormal small intestine morphology ( J:162678 )

• tamoxifen-treated mice exhibit atrophy of the small intestine compared with wild-type mice

abnormal small intestinal villus morphology ( J:162678 )

• 6 days after tamoxifen treatment, mice exhibit a greater than 80% loss of villus epithelium compared with wild-type mice however, no increase in apoptosis is observed

cellular

abnormal centrosome morphology ( J:162678 )

• tamoxifen-treated mouse embryonic fibroblasts exhibit supernumerary centrosomes compared with similarly treated wild-type cells

abnormal cell nucleus morphology ( J:162678 )

• tamoxifen-treated mouse embryonic fibroblasts stimulated with serum exhibit abnormal nuclei including binuclear, multi-lobed, and donut-shaped nuclei compared to similarly treated wild-type cells

abnormal mitosis ( J:162678 )

• tamoxifen-treated mouse embryonic fibroblasts exhibit a delayed entry into mitosis (G2 delay) compared with similarly treated wild-type mice

• tamoxifen-treated mouse embryonic fibroblasts exhibit mitotic errors including chromatin bridges, failed nuclear segregation, cytokinesis failure, chaotic multipolar spindles, and supernumerary centrosomes compared with similarly treated wild-type cells

decreased fibroblast proliferation ( J:162678 )

• following tamoxifen treatment, mouse embryonic fibroblasts exhibit severely impaired proliferation compared with similarly treated wild-type cells

abnormal small intestinal crypt cell proliferation ( J:162678 )

• tamoxifen-treated mice exhibit reduced cell proliferation with abnormal nuclei and mitotic increased nuclear area compared with wild-type mice