nervous system
• CA1 pyramidal neurons exhibit lower spine density of dendrites compared to in wild-type mice
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• unlike in wild-type mice, Ro 25-6981 fails to inhibit eEPSC
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• NMDAR-eEPSC amplitude is slightly reduced compared to in wild-type mice
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• NMDAR-evoked excitatory postsynaptic current (eEPSC) decay time constants are almost twice as fast as in wild-type mice
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• unlike in wild-type mice, no increase in field excitatory postsynaptic potential (fEPSP) is observed under a protocol designed to evoke modest, subsaturating long term potentiation
• under a protocol designed to produce saturating long term potentiation, mice fail to exhibit a decrease in fEPSP during the first time point compared with similarly treated wild-type mice
• under a long term depression protocol, fEPSP slopes fail to decrease as in similarly treated wild-type mice
• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice
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• unlike in wild-type mice, no increase in field excitatory postsynaptic potential (fEPSP) is observed under a protocol designed to evoke modest, subsaturating long term potentiation
• under a protocol designed to produce saturating long term potentiation, mice fail to exhibit a decrease in fEPSP during the first time point compared with similarly treated wild-type mice
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• application of the glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC) does not produce long term depression unlike in wild-type mice
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• under a long term depression protocol, fEPSP slopes fail to decrease as in similarly treated wild-type mice
• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice
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behavior/neurological
• in a T maze, mice exhibit fewer spontaneous alternations compared with wild-type mice
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• mice exhibit less freezing during retrieval of a trace-conditioned fear memory compared with similarly treated wild-type mice
• however, mice exhibit normal delay-conditioned fear memory and freezing during conditioning of before tone presentation
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• in a hidden platform Morris water maze, mice exhibit a longer latency in finding a hidden platform and spend less time in a platform quadrant compared with wild-type mice
• however, mice exhibit normal performance during visible platform training
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• in a hidden platform Morris water maze but not when the platform is visible
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• in a hidden platform Morris water maze, mice swim slower than wild-type mice
• however, swimming is normal when the platform is visible
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homeostasis/metabolism
• unlike in wild-type mice, Ro 25-6981 fails to inhibit evoked excitatory postsynaptic current (eEPSC)
• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice
• application of tPDC does not produce long term depression unlike in wild-type mice
• however, DL-AP-5 inhibits eEPSP as in wild-type mice
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