Phenotypes Associated with This Genotype

Genotype
MGI:4442918

• Allelic Composition: Pclo^tm3.2Sud/Pclo^tm3.2Sud
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:159311 )

• 20% of mice die by P60

premature death ( J:159311 )

• 20% of mice die by P60

nervous system

nervous system phenotype ( J:159311 )

N • regardless of treatment with an shRNA Bsn lentivirus, cultured neurons exhibit normal excitatory and inhibitory synaptic transmission, short-term synaptic plasticity, and ready releasable pool

abnormal synaptic vesicle number ( J:159311 )

• following treatment with an shRNA Bsn lentivirus, neurons exhibit decreased synaptic vesicle clustering, number of vesicles in nerve terminals, overall density of vesicles per terminal area, overall vesicle numbers, the number of vesicles close to the active zone, and docked at the active zone compared to in wild-type cells

• following treatment with an shRNA Bsn lentivirus, neurons exhibit an increase in postsynaptic density compared with wild-type cells

• however, mice exhibit normal size of presynaptic terminals and synaptic vesicles

increased synaptic depression ( J:159311 )

• during a 15-Hz stimulus train, mice exhibit a small increase in depression at later stages compared with similarly treated wild-type mice

• however, mice exhibit normal paired-pulse facilitation, post-tetanic potentiation, and short-term plasticity is normal

growth/size/body

decreased body size ( J:159311 )