Phenotypes Associated with This Genotype

Genotype
MGI:4430568

• Allelic Composition: Cd7^tm1Bfh/Cd7^tm1Bfh
• Genetic Background: B6.129-Cd7^tm1Bfh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:48758 )

N • homozygotes exhibit normal total serum Ig levels and serum Ig subclass levels relative to wild-type controls

N • no defects in T-dependent anti-tetanus toxoid B cell Ig responses are observed relative to wild-type controls

N • no defects in splenocyte or thymocyte proliferation are observed in mitogen-stimulated or TCR-mediated T cell activation assays relative to wild-type controls

N • at 4 months of age, homozygotes exhibit normal NK cell functional activity, as assessed by the ability to lyse YAC target cells

increased thymocyte number ( J:48758 )

• at 3 months of age, homozygotes display a 60% increase in thymocyte number due to a selective and transient increase in the number of double-positive thymocytes

increased double-positive T cell number ( J:48758 )

• at 3 months of age, homozygotes display a 60% increase in the number of CD4⁺ CD8⁺ T cells relative to wild-type controls

• however, this increase in only transient as normal numbers of double-positive T cell numbers are detected at both younger (4 weeks) and older ages (6 and 12 months)

decreased cytotoxic T cell cytolysis ( J:48758 )

• at 3 months of age, homozygotes display a lower splenic Ag-induced MHC class I-restricted CTL activity against OVA-transfected target cells than wild-type controls

decreased interferon-gamma secretion ( J:48758 )

• production of supernatant IFN-gamma is significantly reduced in tetanus toxoid-immunized mutant splenocytes after restimulation in vitro with tetanus toxoid

• however, normal IFN-gamma production is observed in mutant bulk splenocytes in response to PHA, ConA, CD3 + CD28, and OVA peptide relative to wild-type controls

hematopoietic system

increased thymocyte number ( J:48758 )

• at 3 months of age, homozygotes display a 60% increase in thymocyte number due to a selective and transient increase in the number of double-positive thymocytes

increased double-positive T cell number ( J:48758 )

• at 3 months of age, homozygotes display a 60% increase in the number of CD4⁺ CD8⁺ T cells relative to wild-type controls

• however, this increase in only transient as normal numbers of double-positive T cell numbers are detected at both younger (4 weeks) and older ages (6 and 12 months)

decreased cytotoxic T cell cytolysis ( J:48758 )

• at 3 months of age, homozygotes display a lower splenic Ag-induced MHC class I-restricted CTL activity against OVA-transfected target cells than wild-type controls

endocrine/exocrine glands

increased thymocyte number ( J:48758 )

• at 3 months of age, homozygotes display a 60% increase in thymocyte number due to a selective and transient increase in the number of double-positive thymocytes