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Phenotypes Associated with This Genotype
Genotype
MGI:4421697
Allelic
Composition
Lyve1tm1.1(EGFP/cre)Cys/Lyve1+
Sphk1tm2Cgh/Sphk1tm2Cgh
Sphk2tm1.1Cgh/Sphk2tm1.1Cgh
Genetic
Background
involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyve1tm1.1(EGFP/cre)Cys mutation (1 available); any Lyve1 mutation (9 available)
Sphk1tm2Cgh mutation (1 available); any Sphk1 mutation (2 available)
Sphk2tm1.1Cgh mutation (1 available); any Sphk2 mutation (11 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Impaired lymphocyte egress in Lyve1tm1.1(EGFP/cre)Cys/Lyve1+ Sphk1tm2Cgh/Sphk1tm2Cgh Sphk2tm1.1Cgh/Sphk2tm1.1Cgh mice

immune system
N
• lymphatics of mutants can support bone marrow-derived dendritic cell migration
• egress of lymphocytes from lymph nodes is impaired
• lymphatics of mutants can support bone marrow-derived dendritic cell migration
• a decrease in lymphocyte numbers is detected in the circulation and spleen
• markedly reduced in lymph isolated from cisterna chyli
• markedly reduced in lymph isolated from cisterna chyli; reduction in T cells consistently seen in lymphocyte compartment of lymph nodes, but Peyer's patches show accumulation of naive T cells
• lymphocyte number is reduced in the spleen
• accumulation of naive T cells in Peyer's patches is observed concomitant with reduced T cell numbers in the lymph nodes
• lymphatic sinuses show deficiency of lymphocytes
• Lyve1-positive sinuses appear collapsed compared to control sinuses, which are extended with cells
• lymphatic vessels in the trachea appear tortuous and ragged with occasional sprouts, in contrast to the smooth tubular structure in control animals; lymphatics within the diaphragm have similar altered morphology
• cell-cell junctions of initial lymphatics in the diaphragm are more poorly defined, being made up of fewer or more diffuse Cdh5 (VE-cadherin) +ve "buttons"

hematopoietic system
• egress of lymphocytes from lymph nodes is impaired
• lymphatics of mutants can support bone marrow-derived dendritic cell migration
• a decrease in lymphocyte numbers is detected in the circulation and spleen
• markedly reduced in lymph isolated from cisterna chyli
• markedly reduced in lymph isolated from cisterna chyli; reduction in T cells consistently seen in lymphocyte compartment of lymph nodes, but Peyer's patches show accumulation of naive T cells
• lymphocyte number is reduced in the spleen

cellular
• egress of lymphocytes from lymph nodes is impaired
• lymphatics of mutants can support bone marrow-derived dendritic cell migration


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/09/2019
MGI 6.14
The Jackson Laboratory