Phenotypes Associated with This Genotype

Genotype
MGI:4420999

• Allelic Composition: Tg(Camk2a-tTA)1Mmay/0; Tg(tetO-LRRK2*G2019S)E3Cai/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:156512 )

• starting at 1 year, mice appear to gain less body weight than non-transgenic and Tg(tetO-LRRK2*G2019S)E3Cai single mutant mice, but are not different from Tg(Camk2a-tTA)1Mmay animals

behavior/neurological

behavior/neurological phenotype ( J:156512 )

N • mice show normal performance in the rotarod test

hyperactivity ( J:156512 )

• mice show significantly increased ambulatory activities starting at 12 months of age; at 12 months mice show a trend to increased rearing activities but this does not reach statistical significance

nervous system

nervous system phenotype ( J:156512 )

N • no significant changes in neuron counts are observed compared to non-transgenic controls at the frontal cortex or dorsal striatum in 6- or 20-month old mice

N • no increase in reactive astrocytosis or microglial activation are detected in the striatum or cortex at 20 months

N • no alpha-synuclein accumulation is observed in neurons at 20 months

abnormal neuron morphology ( J:156512 )

• the Golgi complex is drastically altered in neurons at 1 month, appearing thinner and fragmented

• at 6 months fragmentation of the cis-Golgi apparatus is similar to that in LRRK2WT and A53T/LRRK2 neurons

• at 1 month, the medial/trans-Golgi in neurons is significantly altered

abnormal nervous system physiology ( J:156512 )

• brain homogenates moderately elevated levels of ubiquitinated proteins at 18 months compared to non-transgenic controls