Phenotypes Associated with This Genotype

Genotype
MGI:4399130

• Allelic Composition: Zap70^tm2.1Weis/Zap70^tm2.1Weis
• Genetic Background: C.129S4-Zap70^tm2.1Weis

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:154166 )

N • despite defects in T cell selection, mice do not display signs of autoimmunity and do not develop substantial amounts of anti-double-stranded DNA (dsDNA) autoantibodies after zymosan stimulation.

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

abnormal T cell clonal deletion ( J:154166 )

• defective clonal deletion of Vbeta5 and Vbeta11 thymocytes from endogenous viral superantigens, Mtv-8 and Mtv-9

abnormal positive T cell selection ( J:154166 )

• numbers of the HSA^lo TCRbeta^hi cells are substantially decreased, as compared with thymocytes from wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD4 single positive thymocytes are profoundly reduced, and fewer mature CD4+ T cells are present in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD8 single positive thymocytes are profoundly reduced, and fewer mature CD8+ T cells are present in the periphery

increased memory T cell number ( J:154166 )

• increase in percentages of memory CD4 + T cells

decreased regulatory T cell number ( J:154166 )

• reduction in both thymic regulatory T-cells cell frequency as well as absolute regulatory T-cell numbers

increased activated T cell number ( J:154166 )

• increase in percentage of CD45RB^lo CD4+ T cells

abnormal splenic cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

abnormal regulatory T cell physiology ( J:154166 )

• regulatory T-cells are much less efficient at suppressing proliferation of wild-type naive T cells

abnormal lymph node cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

increased interleukin-17 secretion ( J:154166 )

• the percentages of IL-17 producing cells are 6- fold higher than in the wild-type controls

decreased interleukin-2 secretion ( J:154166 )

• IL-2 production after CD3 stimulation is greatly reduced

hematopoietic system

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

abnormal T cell clonal deletion ( J:154166 )

• defective clonal deletion of Vbeta5 and Vbeta11 thymocytes from endogenous viral superantigens, Mtv-8 and Mtv-9

abnormal positive T cell selection ( J:154166 )

• numbers of the HSA^lo TCRbeta^hi cells are substantially decreased, as compared with thymocytes from wild-type mice

decreased CD4-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD4 single positive thymocytes are profoundly reduced, and fewer mature CD4+ T cells are present in the periphery

decreased CD8-positive, alpha-beta T cell number ( J:154166 )

• absolute numbers of CD8 single positive thymocytes are profoundly reduced, and fewer mature CD8+ T cells are present in the periphery

increased memory T cell number ( J:154166 )

• increase in percentages of memory CD4 + T cells

decreased regulatory T cell number ( J:154166 )

• reduction in both thymic regulatory T-cells cell frequency as well as absolute regulatory T-cell numbers

increased activated T cell number ( J:154166 )

• increase in percentage of CD45RB^lo CD4+ T cells

abnormal splenic cell ratio ( J:154166 )

• significant decrease in the absolute number of CD4 and CD8 single positive subsets

abnormal regulatory T cell physiology ( J:154166 )

• regulatory T-cells are much less efficient at suppressing proliferation of wild-type naive T cells

endocrine/exocrine glands

abnormal thymus cell ratio ( J:154166 )

• significant decrease in the relative percentage and absolute number of CD4 and CD8 single positive subsets

• however, total thymic cellularity is only mildly decreased

abnormal thymocyte activation ( J:154166 )

• an attenuated TCR-induced increase in cytoplasmic free calcium in thymocytes and peripheral T cells

cellular

decreased T cell proliferation ( J:154166 )

• poor proliferative responses to TCR and CD28 stimulation; however, the T cells partially overcome the proliferative defect in response to higher doses