Phenotypes Associated with This Genotype

Genotype
MGI:3845392

• Allelic Composition: Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw; Cdc25b^tm1Pjd/Cdc25b^tm1Pjd; Cdc25c^tm1Hpw/Cdc25c^tm1Hpw; Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Shortened villi in the small intestine of Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺ (TKO) mice

mortality/aging

premature death ( J:145768 )

• adult mice die within 1 week of initial 4-hydroxytamoxifen (OHT) injection

growth/size/body

weight loss ( J:145768 )

• adult mice exhibit significant weight loss (20%) within 1 week of initial tamoxifen injection

digestive/alimentary system

abnormal small intestine crypts of Lieberkuhn morphology ( J:145768 )

• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth

• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed

• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts

• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells

abnormal small intestinal villus morphology ( J:145768 )

• villi are lined with shorter, less-dense enterocytes in proximal regions; complete loss of distal villi is observed in some areas

decreased small intestinal villus height ( J:145768 )

• significant loss of villus height in proximal and distal regions is observed in mutants after OHT treatment

decreased small intestine length ( J:145768 )

• 6 days following the final of 3 consecutive injections of tamoxifen, length of small intestine is shortened by 40% compared to controls

endocrine/exocrine glands

abnormal small intestine crypts of Lieberkuhn morphology ( J:145768 )

• profound cell atrophy in the crypts is observed in OHT-treated mice; crypts are >40% smaller in mutants than Cdc25a-single knockout controls, with the crypt width being decreased more significantly than the depth

• a 70% loss of epithelial cells per crypt is detected, but no difference in mature Paneth cells is observed

• decreased proliferation of epithelial cells is observed, with no significant increase in apoptotic cell numbers; this results in premature differentiation of cell progenitors below Paneth cell compartments in crypts

• crypts have increased numbers of cells exhibiting early differentiation with no crypt base columnar (CBC) cells