Phenotypes Associated with This Genotype

Genotype
MGI:3844021

• Allelic Composition: Myh9^tm1.1Gac/Myh9^tm1.1Gac; Tg(Pf4-icre)Q3Rsko/0
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:148233 )

• during bleed time experiments, mice die within 20 minutes if not cauterized manually

hematopoietic system

abnormal platelet morphology ( J:148233 )

• platelets are twice the size as in wild-type mice

thrombocytopenia ( J:148233 )

• the number of platelets is 40% of wild-type

• however, in vitro platelet half-life is normal

abnormal platelet shape ( J:148233 )

• some ovoid platelets are mixed in with the normal population unlike in wild-type mice

• following activation with thrombin or U46619, platelets fail to undergo spheration unlike similarly treated wild-type platelets

• activated platelets exhibit very little shape change unlike wild-type platelets

abnormal platelet physiology ( J:148233 )

• platelets fail to exhibit retraction after thrombin activation unlike wild-type platelets

• at low concentrations of thrombin and U46619, platelets exhibit decreased aggregation compared to wild-type platelets

• following activation with thrombin or U46619, platelets fail to undergo spheration unlike similarly treated wild-type platelets

• serotonin release from dense granules is decreased after activation with U46619 or low concentrations of thrombin compared to similarly treated wild-type platelets

• activated platelets exhibit very little shape change unlike wild-type platelets

• activated platelets fail to form stress fibers unlike wild-type platelets

• however, platelet aggregation does occur

homeostasis/metabolism

abnormal blood coagulation ( J:148233 )

• in vitro under flow conditions and in vivo, thrombus organization is impaired compared to in wild-type mice

abnormal platelet physiology ( J:148233 )

• platelets fail to exhibit retraction after thrombin activation unlike wild-type platelets

• at low concentrations of thrombin and U46619, platelets exhibit decreased aggregation compared to wild-type platelets

• following activation with thrombin or U46619, platelets fail to undergo spheration unlike similarly treated wild-type platelets

• serotonin release from dense granules is decreased after activation with U46619 or low concentrations of thrombin compared to similarly treated wild-type platelets

• activated platelets exhibit very little shape change unlike wild-type platelets

• activated platelets fail to form stress fibers unlike wild-type platelets

• however, platelet aggregation does occur

increased bleeding time ( J:148233 )

• mice exhibit increased bleed time compared with wild-type mice

• during bleed time experiments, mice die within 20 minutes if not cauterized manually