Phenotypes Associated with This Genotype

Genotype
MGI:3843508

• Allelic Composition: Tcrb^tm1(Tcrb)Chb/Tcrb^tm1Slek
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell morphology ( J:147947 )

• there is a decreased percentage of alpha-beta T cells expressing T Cell receptors (TCR) that are partially encoded Vbeta5 or Vbeta8 gene segments

abnormal T cell receptor beta chain V(D)J recombination ( J:147947 )

• in T cells, complete VbetaDJbeta exon rearrangement occurs more frequently (69%) on the Tcrb^tm1(Tcrb)Chb allele than on the Tcrb^tm1Slek allele (31%)

• sequencing of T cell hybridomas suggest there is both an increase in rearrangements on the Tcrb^tm1(Tcrb)Chb allele and a preferential selection of thymocytes with in-frame VbetaDJbeta rearrangements on this allele

abnormal double-negative T cell morphology ( J:147947 )

• the frequency of DN3 thymocytes is slightly decreased while the frequency of DN4 thymocytes is slightly increased

decreased CD8-positive, alpha-beta T cell number ( J:147947 )

• frequency of CD8⁺ T cells in the spleen is slightly decreased

increased single-positive T cell number ( J:147947 )

• there is an increased frequency of single-positive thymocytes (both CD4⁺ and CD8⁺)

hematopoietic system

abnormal T cell morphology ( J:147947 )

• there is a decreased percentage of alpha-beta T cells expressing T Cell receptors (TCR) that are partially encoded Vbeta5 or Vbeta8 gene segments

abnormal T cell receptor beta chain V(D)J recombination ( J:147947 )

• in T cells, complete VbetaDJbeta exon rearrangement occurs more frequently (69%) on the Tcrb^tm1(Tcrb)Chb allele than on the Tcrb^tm1Slek allele (31%)

• sequencing of T cell hybridomas suggest there is both an increase in rearrangements on the Tcrb^tm1(Tcrb)Chb allele and a preferential selection of thymocytes with in-frame VbetaDJbeta rearrangements on this allele

abnormal double-negative T cell morphology ( J:147947 )

• the frequency of DN3 thymocytes is slightly decreased while the frequency of DN4 thymocytes is slightly increased

decreased CD8-positive, alpha-beta T cell number ( J:147947 )

• frequency of CD8⁺ T cells in the spleen is slightly decreased

increased single-positive T cell number ( J:147947 )

• there is an increased frequency of single-positive thymocytes (both CD4⁺ and CD8⁺)