Phenotypes Associated with This Genotype

Genotype
MGI:3839952

• Allelic Composition: Mre11a^tm1Dof/Mre11a^tm2.1Dof
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

cellular phenotype ( J:147247 )

N • cre-treated mouse embryonic fibroblasts exhibit normal G2/M checkpoint activation following exposure to ionizing radiation

increased cellular sensitivity to ionizing radiation ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit increased cellular sensitivity to ionizing radiation compared to similarly treated heterozygous cells that is not as severe as in Lig4^tm1Fwa cells

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in similarly treated heterozygous mice

decreased cell proliferation ( J:147247 )

• cre-exposed mouse embryonic fibroblasts exhibit decreased growth rates by the second passage and no increase in numbers beyond passage three unlike similarly treated heterozygous cells

abnormal DNA repair ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in wild-type mice

chromosomal instability ( J:147247 )

• cre-exposed mouse embryonic fibroblasts (MEFs) exhibit chromosomal instability including chromosome or chromatid fragments, chromosome breaks or gaps in a single chromatid, occasional radial structures, and dicentrics unlike in similarly treated heterozygous cells

• following exposure to gamma radiation, cre-exposed MEFs fail to exhibit a downward trend in frequency of chromosomal anomalies unlike similarly treated heterozygous cells

induced chromosome breakage ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit hypersensitivity to aphidicolin compared to similarly treated heterozygous cells

homeostasis/metabolism

abnormal DNA repair ( J:147247 )

• cre-treated mouse embryonic fibroblasts exhibit defective DNA repair following treatment with ionizing radiation compared to in wild-type mice