Phenotypes for Isl1 Tg(Six3-cre)69Frty MGI:3838015 MGI Mouse

vision/eye phenotype ( J:146349 )

N	• the number and organization of the photoreceptor cells in the outer nuclear layer are normal

increased retina apoptosis ( J:146349 )

• at E17.5, the number of cells undergoing apoptosis in the retina is greater than in wild-type retinas

• however, retinal apoptosis at E14.5 is normal

decreased amacrine cell number ( J:146349 )

• at P10, amacrine cells are reduced compared to in wild-type mice

• at P16, cholinergic amacrine cells are completely lost unlike in wild-type mice

abnormal retina bipolar cell morphology ( J:146349 )

• at P10, the number of bipolar cells is reduced compared to in wild-type mice

• at P16, cone on-bipolar and rod bipolar cells are completely lost unlike in wild-type mice

abnormal retina ganglion cell morphology ( J:146349 )

• at E17.5, the number of Pou4f2+ retinal ganglion cells in the neuroblast layer is greater than in wild-type mice

• however, mice exhibit normal retinal ganglion cell development and numbers at E14.5

decreased retina ganglion cell number ( J:146349 )

• 28% Pou4f2+ retinal ganglion cells of wild-type at P5

• 5% Pou4f2+ retinal ganglion cells of wild-type at P16

abnormal optic nerve morphology ( J:146349 )

• at P16, the optic nerve is thin and severely disrupted compared to in wild-type mice within thin axons that are not properly myelinated

optic nerve degeneration ( J:146349 )

• axons within the optic never exhibit degeneration unlike in wild-type mice

microphthalmia ( J:146349 )

• at P16

thin retina inner nuclear layer ( J:146349 )

• the inner nuclear layer is half as thick as in wild-type mice due to a decrease in cell numbers within the layer

thin retina inner plexiform layer ( J:146349 )

abnormal retina outer plexiform layer morphology ( J:146349 )

• the margins of the outer plexiform layer are ragged unlike in wild-type mice